Positve control tissue options (tissue types used may vary with availability):
Tonsil tissue:
(+) Human tonsil or appendix
(-) Liver or cervix (tissue type may vary with availability)
More than 20% of germinal centre B-cells should show a weak to moderate nuclear staining reaction, while less than 10% of the mantle zone B-cells should be demonstrated in tonsil.
Colon cancer tissue:
(+) Colon cancer
(-) Liver or cervix (tissue type may vary with availability)
Appendix tissue: Dispersed epithelial cells in the basal parts of the crypts should show a weak to moderate nuclear staining reaction, while the luminal epithelial cells are negative.
Pack size:
25 slide pack(24 unstained +1 stained),
100 slide pack(98 unstained +2 stained, 1st &100th sections stained)
P53 Control Slides
Fixative: 10% Neutral buffered formalin
Embedding: Paraffin
Thickness: 4 μm
Mounting: Mounted on positively charged slides made of pure white glass; air dried
Storage conditions: RT, light-deprived, humidity-controlled
Deparaffinize and rehydrate before use.
INTENDED USE: P53 positive control slides are intended for use as positive controls for immunohistochemical (IHC) staining. Positive tissue controls should only be utilized for monitoring the correct performance of processed tissues and test reagents, rather than as an aid in the interpretation of samples. If the positive tissue controls fail to demonstrate positive staining, results with the test specimens should be considered invalid.